The Bruker Ascend 700 MHZ Nuclear Magnetic Resonance Spectrometer (NMR) with cryo-probe is ideal for structural biology research and materials research applications. Highly sensitive for small compounds. It is ideal for structure elucidation at atomic resolution and protein binding studies. For more Bruker Ascend 700 MHZ NMR information click here!
Nuclear Magnetic Resonance (NMR) spectroscopy is an analytical technique that uses radiofrequencies to detect atomic nuclei (e.g. 1H, 13C, 15N and 31P) in molecules. Together with X-ray crystallography, NMR spectroscopy is one of the two main methods to study molecular structures in detail at the atomic level. At the department of Biochemistry, Hans Ippel (NMR spectroscopist@Maastricht University) operates a Bruker Avance III HD NMR spectrometer operating at a frequency of 700 MHz and equipped with a cryogenically cooled probe. The system is used to analyze organic molecules, proteins, peptides, synthetic polymers, but also complete cells and whole urine and blood samples can be measured for identification and quantification of metabolites, all in a non-destructive way. The NMR data contributes significantly to the understanding of the molecular basis of cardiovascular diseases like atherosclerosis, as well as on the design and discovery of novel pharmaceutical agents. Typical work cases are solving three-dimensional protein structures (e.g. on TFPI, chemokines RANTES and Platelet-Factor 4, and tick evasins) including assesment of their specific binding interactions by means of 2D and 3D NMR spectra. Other users from MUMC are using NMR spectroscopy for metabolic profiling of various types of biofluids, while companies like DSM Resolve, Cristal Therapeutics, Matisse Pharmaceuticals and OKKLO BV mainly apply our NMR tools to check the structural integrity and correctness of functional molecular targets. We can assist researchers to acquire spectra by their own and/or help in the analysis of the results. Our important scientific motto hangs on the wall as art: “There is no Chaos, only Structure”, because NMR is well suited to study the beauty of structural organization in Nature.
NMR spectrometry, UNS50 room 4.301
Telephone: +31 (0)6 22028425